Coding
INT-6N

Part:BBa_K1433015:Design

Designed by: Chaofan Zhang   Group: iGEM14_ZJU-China   (2014-10-09)


Promoter-6N-gp35-T


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 55
    Illegal NheI site found at 252
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 526
    Illegal XhoI site found at 613
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1165
    Illegal NgoMIV site found at 1252
    Illegal AgeI site found at 302
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1360


Design Notes

no


Source

Derive from 2014 Distribution Kit.

References

  1. Bonnet J, Subsoontorn P, Endy D. Rewritable digital data storage in live cells via engineered control of recombination directionality[J]. Proceedings of the National Academy of Sciences, 2012, 109(23): 8884-8889.
  2. Siuti P, Yazbek J, Lu T K. Synthetic circuits integrating logic and memory in living cells[J]. Nature biotechnology, 2013, 31(5): 448-452.